4pi-microscopy with 1-photon Excitation
نویسندگان
چکیده
Over the last years two-photon excitation 4Pi-microscopy with an axial resolution of ~100 nm has been demonstrated in multiple biological applications [1-3]. The artefact-free presentation of the recorded structures has been possible because of the contiguous optical transfer function (OTF), which allows reliable image deconvolution. The use of two-photon excitation (2PE) has been critical to suppress the artefact-producing side-lobes of the 4Pi-point spread function.
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